Plasmid

Part:BBa_K1391113:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-30)

pEXPR hEF1a:PirB_TCS_Gal4VP16


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 5758
    Illegal EcoRI site found at 7249
    Illegal EcoRI site found at 8763
    Illegal EcoRI site found at 8804
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 6838
    Illegal PstI site found at 9341
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 5758
    Illegal EcoRI site found at 7249
    Illegal EcoRI site found at 8763
    Illegal EcoRI site found at 8804
    Illegal NheI site found at 3210
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 6838
    Illegal PstI site found at 9341
    Illegal NotI site found at 9646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 5758
    Illegal EcoRI site found at 7249
    Illegal EcoRI site found at 8763
    Illegal EcoRI site found at 8804
    Illegal BglII site found at 4818
    Illegal BglII site found at 8884
    Illegal BamHI site found at 2854
    Illegal BamHI site found at 3328
    Illegal BamHI site found at 5424
    Illegal BamHI site found at 5471
    Illegal BamHI site found at 5567
    Illegal BamHI site found at 9841
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 5217
    Illegal XhoI site found at 8519
    Illegal XhoI site found at 9626
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 5758
    Illegal EcoRI site found at 7249
    Illegal EcoRI site found at 8763
    Illegal EcoRI site found at 8804
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 6838
    Illegal PstI site found at 9341
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 5758
    Illegal EcoRI site found at 7249
    Illegal EcoRI site found at 8763
    Illegal EcoRI site found at 8804
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 6838
    Illegal PstI site found at 9341
    Illegal NgoMIV site found at 2297
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4024
    Illegal NgoMIV site found at 4952
    Illegal AgeI site found at 3456
    Illegal AgeI site found at 4330
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8438
    Illegal BsaI.rc site found at 1121
    Illegal SapI site found at 38
    Illegal SapI site found at 3214
    Illegal SapI site found at 10073
    Illegal SapI.rc site found at 7576


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

Mouse, tobacco etch virus

References